Journal: Dentistry Journal

Article Title: In Vivo Evaluation of Regenerative Osteogenic Potential Using a Human Demineralized Dentin Matrix for Dental Application

doi: 10.3390/dj12030076

Figure Lengend Snippet: Representative histological images of (H&E) ( A , D , G , J ), Masson’s trichrome ( B , E , H , K ) and osteocalcin immune staining ( C , F , I , L ) are shown at 4 weeks post-treatment. Coronal sections through the mid-point of the defects were prepared from decalcified specimens. Fibrous connective tissues were seen to be mostly filled in the defect areas in the control group ( A – C ). The group treated with SA hydrogel as the only grafted material showing fibrous and adipose tissue ( D – F ). lacuna spaces were observed in the newly formed lamellar bone in NHH with a wide bone marrow space ( G – I ). DDMH-treated groups showed considerable new bone trabeculae formation ( J – L ). BT: bone trabeculae; FT: fibrous tissue. Arrows represent MT- and OCN-positive reactions. Scale bar: 100 μm.

Article Snippet: Subsequently, the samples were embedded in paraffin; and after staining with hematoxylin and eosin (H&E), Masson’s trichrome (MT) (Cat# HT15, Sigma Aldrich), and immunohistochemical staining with a polyclonal anti-rabbit osteocalcin antibody (OCN; Cat# GB11233, diagnostic biosystems) at a 1:150 dilution, histological examination was conducted using an Olympus BX-51 optical microscope (Olympus Co., Tokyo, Japan).

Techniques: Staining, Control

Journal: Dentistry Journal

Article Title: In Vivo Evaluation of Regenerative Osteogenic Potential Using a Human Demineralized Dentin Matrix for Dental Application

doi: 10.3390/dj12030076

Figure Lengend Snippet: Representative histological images of (H&E) ( A , D , G , J ), Masson’s trichrome ( B , E , H , K ) and osteocalcin immune staining ( C , F , I , L ) are shown at 8 weeks post-treatment. Rather than the newly created bone, the defect locations in the control group were found to be primarily filled with fibrous connective tissues and adipose tissue ( A – C ). Circular new bony islands with the presence of early woven bone were observed in the group treated with SA as the only grafted material ( D – F ). Newly formed lamellar bone was observed in NHH with a wide bone marrow space ( G – I ). In contrast, DDMH-treated groups showed considerable new bone formation at 8 weeks with relatively narrow marrow spaces ( J – L ). BT means bone trabeculae; FT: fibrous tissue. Arrows represent MT- and OCN-positive reactions. Scale bar: 100 μm.

Article Snippet: Subsequently, the samples were embedded in paraffin; and after staining with hematoxylin and eosin (H&E), Masson’s trichrome (MT) (Cat# HT15, Sigma Aldrich), and immunohistochemical staining with a polyclonal anti-rabbit osteocalcin antibody (OCN; Cat# GB11233, diagnostic biosystems) at a 1:150 dilution, histological examination was conducted using an Olympus BX-51 optical microscope (Olympus Co., Tokyo, Japan).

Techniques: Staining, Control

Journal: International Journal of Nanomedicine

Article Title: Hollow Hydroxyapatite Microspheres Loaded with rhCXCL13 to Recruit BMSC for Osteogenesis and Synergetic Angiogenesis to Promote Bone Regeneration in Bone Defects

doi: 10.2147/IJN.S408905

Figure Lengend Snippet: Analysis of osteogenic differentiation in BMSCs co-cultured with scaffolds. ( A ) Quantitative RT-qPCR analysis of osteogenic gene expression (Runx2, ALPL, OCN) in BMSCs co-cultured with each group. GAPDH was used as housekeeping genes. The results were expressed as mean ± SD. ( B ) Relative protein expression of RUNX2 and OCN in BMSCs co-cultured with various treatment groups. ( C ) Representative images of Alizarin red-stained BMSCs co-cultured with various scaffolds for 21 days in 6-well plates (scale bar = 100μm). ( D ) Quantitative analysis of Alizarin Red Staining in BMSCs co-cultured with various treatment groups. Statistical analysis was performed using ANOVA and LSD tests, with results expressed as mean ± SD (*p < 0.05, **p < 0.01, ***p < 0.001, n=3).

Article Snippet: After the washing, membranes were incubated with rabbit anti-RUNX2 (Abclonal; 1:1000), rabbit anti-OCN (Abclonal; 1:1000), and rabbit anti-GAPDH (Abclonal; 1:1000) antibodies at 4◦C overnight.

Techniques: Cell Culture, Quantitative RT-PCR, Expressing, Staining

Journal: International Journal of Nanomedicine

Article Title: Hollow Hydroxyapatite Microspheres Loaded with rhCXCL13 to Recruit BMSC for Osteogenesis and Synergetic Angiogenesis to Promote Bone Regeneration in Bone Defects

doi: 10.2147/IJN.S408905

Figure Lengend Snippet: Immunofluorescence Staining Analysis of Osteogenic Differentiation in BMSCs Co-Cultured with Scaffolds. ( A ) Immunofluorescence staining of BMSCs (COLI, BMP-2, RUNX2, and OCN) when co-cultured with scaffolds (( A and B ). ( B ) Quantitative analysis of immunofluorescence staining for COLI, BMP-2, RUNX2, and OCN performed by Image J. ( a1, a2, b1 and b2 ). Statistical analysis was performed using ANOVA and LSD tests, with results expressed as mean ± SD (*p<0.05, **p<0.01, ***p<0.001, n=3).

Article Snippet: After the washing, membranes were incubated with rabbit anti-RUNX2 (Abclonal; 1:1000), rabbit anti-OCN (Abclonal; 1:1000), and rabbit anti-GAPDH (Abclonal; 1:1000) antibodies at 4◦C overnight.

Techniques: Immunofluorescence, Staining, Cell Culture

Journal: International Journal of Nanomedicine

Article Title: Hollow Hydroxyapatite Microspheres Loaded with rhCXCL13 to Recruit BMSC for Osteogenesis and Synergetic Angiogenesis to Promote Bone Regeneration in Bone Defects

doi: 10.2147/IJN.S408905

Figure Lengend Snippet: Heat Map, Protein-Protein Interaction (PPI) Network, and KEGG Up-Pathway Enrichment Analyses in Various Treatment Groups. ( A ) Heat Map of differentially expressed genes related to cellular activity, osteogenesis, angiogenesis, and BMSC recruitment in different groups comparisons. ( B1 and B2 ) PPI analysis revealing the function of DEG-encoded proteins in different groups. ( C1-C3 ) KEGG up-pathway enrichment analysis in different groups comparisons. The red symbol in ( C1 ) indicates that the KEGG up-pathway enrichment analysis of differentially expressed genes mainly concentrated in the PI3K-AKT signaling pathway. ( D ) Changes in the relative gene expression levels of RUNX2 and OCN after treatment with PI3K-AKT inhibitor BEZ235 in BMSCs from all groups. ( E ) Representative alizarin red staining image of BMSCs co-cultured with rhCXCL13-HHM/CS scaffold after 21 days of osteogenic differentiation treated with PI3K-AKT inhibitor BEZ235 (scale bar = 100μm, n=3). ( F ) Quantitative analysis of alizarin red staining of BMSCs in different treatment groups (***p<0.001, n=3).

Article Snippet: After the washing, membranes were incubated with rabbit anti-RUNX2 (Abclonal; 1:1000), rabbit anti-OCN (Abclonal; 1:1000), and rabbit anti-GAPDH (Abclonal; 1:1000) antibodies at 4◦C overnight.

Techniques: Activity Assay, Expressing, Staining, Cell Culture